Details

Project TitleA Stable, Immortalized Cell Line for Articular Cartilage Production and Repair
Track Code2014-106
Short Description

Dr. Martin and his lab at the University of Iowa have developed the ChondroNT cell which is created by transducing primary human chondrocytes with genes encoding a telomerase (hTERT) and a transcription factor (Nanog).  Continuous expression of these genes allows for unlimited population growth while retaining the ability to form articular cartilage.  Cells can be grown for over 70 population doublings without exhibiting signs of senescence.  Using this cell line avoids the introduction of oncogenic sequences to overcome growth inhibition.  Thus, ChonroNT is an excellent tool for cartilage tissue repair.

Abstract

Background Information

Cartilage is a flexible connective tissue found in joints ears, nose bronchial tubes and intervertebral discs. Chondrocytes, which are building blocks of cartilage produces a large amount of extracellular matrix which is made up of proteoglycan and elastin fibers. Cartilage can be classified into three categories—elastic, hyaline, and fibrocartilage. Unlike other types of tissues, cartilage does not contain any blood vessels. So, all nutrients and hormone that the tissue requires are delivered by diffusion which is generated by compression or flexion of the tissue. Because of their reliance on diffusion, cartilage grows and repairs much more slowly compared to other tissue types. Cartilage health is a growing industry. NIAMS, predicts that by 2030 20% of Americans-about 70 million people-will have passed their 65th birthday and will be at risk for osteoarthritis.

Technology Summary

Dr. Martin and his lab at the University of Iowa have developed the ChondroNT cell which is created by transducing primary human chondrocytes with genes encoding a telomerase (hTERT) and a transcription factor (Nanog). Continuous expression of these genes allows for unlimited population growth while retaining the ability to form articular cartilage. Cells can be grown for over 70 population doublings without exhibiting signs of senescence. Using this cell line avoids the introduction of oncogenic sequences to overcome growth inhibition. Thus, ChonroNT is an excellent tool for cartilage tissue repair.

Advantages

•Enhanced chondrocyte proliferation without senescence


• Avoid introduction of oncogenic sequences to chondrocytes

 
TagsChondroNT cell line, Martin, articular cartilage, cartilage repair, chondrocyte
 
Posted DateJul 31, 2017 9:56 AM

Licensing Contact

Mihaela D. Bojin, PhD, CLP

Licensing Associate

University of Iowa Research Foundation

2660 UCC

Iowa City, Iowa 52242

Phone: (319) 335-2723

Email: mihaela-bojin@uiowa.edu

University of Iowa Research Foundation

Files

File Name Description
Technical Write Up 2014-106 None Download